|Weapon(s):||Sealed Armament: Izayoi|
|Relatives:||Tsubaki Yayoi (descendant)|
|Novel(s):||BlazBlue: Phase Shift 4|
Ayame looked nearly identical to her descendant, Tsubaki. She had long red hair that was tied back by a black hairband near the tip of the hairs. Her clothes, fashioned by the Izayoi, are the same ones to be donned by her descendant many years later; a creamy white outfit that leaves no skin open to the elements that were accompanied by black boots, the only main difference being that she lacked the gloves that accompanied the outfit.
Although little of her personality is shown, she was shown to be a polite young woman with admiration for Hakumen, like her descendant.
A soldier in the First Great Magic War with a high synchronization rate with Magic Formula. Ayame was the first of the Yayoi family to wield the Sealed Armament: Izayoi and one of the unsung heroes of the Great Dark War, going toe-to-toe with the Black Beast and surviving her encounter against the monstrosity. At the time, she was a mother and presumably married.
She is introduced in Phase Shift 4, she socializes with Hakumen and converses with the Six Heroes before going off to fight the Black Beast. She survived the encounter, but she later died in a tent as the overuse of the Izayoi took her life. It is unknown if she went blind before she died or if her extreme overuse of it against the Black Beast ended up skipping the milestone and instead went straight to taking her life.